DNA and protein microarray printing on silicon nitride ...

DNA and protein microarray printing on silicon nitride waveguide surfaces

DNA and protein microarray printing on silicon nitride waveguide surfaces
Engineering Village 2
2006 Elsevier Inc.
Accession number: 8853687

Title: DNA and protein microarray printing on silicon nitride waveguide surfaces

Authors: Grainger, D.W.1 ; Peng Wu1 ; Hogrebe, P.1

Author affiliation: 1 Dept. of Chem., Colorado State Univ., Fort Collins, CO, USA

Serial title: Biosensors & Bioelectronics

Abbreviated serial title: Biosens. Bioelectron. (UK)

Volume: 21

Issue: 7

Publication date: 15 Jan. 2006

Pages: 1252-63

Language: English

ISSN: 0956-5663

CODEN: BBIOE4

Document type: Journal article (JA)

Publisher: Elsevier

Country of publication: UK

Material Identity Number: N695-2005-015

Abstract: Sputtered silicon nitride optical waveguide surfaces were silanized and modified with a hetero-bifunctional crosslinker to facilitate thiol-reactive immobilization of contact-printed DNA probe oligonucleotides, streptavidin and murine anti-human interleukin-1? capture agents in microarray formats. X-ray photoelectron spectroscopy (XPS) was used to characterize each reaction sequence on the native silicon oxynitride surface. Thiol-terminated DNA probe oligonucleotides exhibited substantially higher surface printing immobilization and target hybridization efficiencies than non-thiolated DNA probe oligonucleotides: strong fluorescence signals from target DNA hybridization supported successful DNA oligonucleotide probe microarray fabrication and specific capture bioactivity. Analogously printed arrays of thiolated streptavidin and non-thiolated streptavidin did not exhibit noticeable differences in either surface immobilization or analyte capture assay signals. Non-thiolated anti-human interleukin-1? printed on modified silicon nitride surfaces reactive to thiol chemistry exhibited comparable performance for capturing human interleukin-1? analyte to commercial amine-reactive microarraying polymer surfaces in sandwich immunoassays, indicating substantial non-specific antibody-surface capture responsible for analyte capture signal. [All rights reserved Elsevier]

Number of references: 49

Inspec controlled terms: biochemistry - biological techniques - DNA - fluorescence - molecular biophysics - optical waveguides - proteins - X-ray photoelectron spectra

Uncontrolled terms: DNA - protein microarray printing - silicon nitride waveguide surfaces - hetero-bifunctional crosslinker - thiol-reactive immobilization - oligonucleotides - streptavidin - X-ray photoelectron spectroscopy - XPS - reaction sequence - silicon oxynitride surface - surface printing immobilization - fluorescence signals - microarray fabrication - polymer surfaces - sandwich immunoassays

Inspec classification codes: A8715M Interactions with radiations at the biomolecular level - A8780 Biophysical instrumentation and techniques - A8715D Physical chemistry of biomolecular solutions; condensed states

Treatment: Experimental (EXP)

Discipline: Physics (A)

DOI: 10.1016/j.bios.2005.05.010

Database: Inspec

Copyright 2006, The Institution of Engineering and Technology

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