Biomolecular sensor based on fluorescence-labeled aptam...

Biomolecular sensor based on fluorescence-labeled aptamer

Biomolecular sensor based on fluorescence-labeled aptamer
15 August 2006
Hiroaki Ozaki, a, , Akifumi Nishihiraa, Masayuki Wakabayashia, Masayasu Kuwaharaa and Hiroaki Sawai
Bioorganic & Medicinal Chemistry Letters
Abstract
Fluorescent DNA probes for l-argininamide were developed by a combination of DNA aptamers and fluorophore?quencher pairs. These molecules were synthesized by a combination of pre- and post-synthetic modification methods. The fluorescence-labeled aptamer could detect l-argininamide specifically. The binding affinities were defined by the binding affinity of the original aptamer to indicate that the end labeling of the aptamer did not influence the affinities.
Graphical abstract
Fluorescence-labeled aptamer for l-argininamide was developed by a combination of DNA aptamer and fluorophore?quencher pairs. The fluorescence intensity of the aptamer was reduced to about one-fifth by the addition of l-argininamide. The binding affinities of the modified aptamers were nearly equal to that of its original aptamer.
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