Use of abasic site-containing DNA for electrochemical SNPs detection

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Use of abasic site-containing DNA for electrochemical SNPs detection
2006
Kotaro Morita1,2, Seiichi Nishizawa1,2 and Norio Teramae1,2
1 Department of Chemistry, Graduate School of Science, Tohoku Univerisity, Aoba-ku, Sendai 980-8578, Japan, 2 CREST, JST, Aoba-ku, Sendai 980-8578, Japan
Nucleic Acids Symposium Series 2006
In combination with an electrode modified with an abasic site-containing DNA duplex, flavins selectively bind to thymine with a useful electrochemical response, and flavins could be successfully utilized for detection of thymine-related mutations. The abasic site provides hydrophobic microenvironments for the hydrogen bond-forming ligands can recognize the specific target base in the DNA duplex, which is detected by the difference in the electrochemical response.
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