Synthesis and property of DNA labeled with fluorescent acridone

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Synthesis and property of DNA labeled with fluorescent acridone
2006
Tomoya Hasegawa1, Atsusi Shoji1,2, Masayasu Kuwahara1,2, Hiroaki Ozaki1 and Hiroaki Sawai1
1 Department of Applied Chemistry, Faculty of Engineering, Gunma University, 1-5-1 Tenjin-chou, Kiryu, Gunma 376-8515, Japan, 2 PRESTO(JST)
Nucleic Acids Symposium Series 2006
Triphosphate of a thymidine analogue bearing acridone was prepared from a modified thymidine nucleotide with a terminal amino group at C5 position and an acridone derivative as a new fluorecsent-tagged nucleotide. The acridone-tagged nucleotide was incorporated into DNA enzymatically during PCR using KOD Dash DNA polymerase. Further, we introduced the acridone derivative into an amino-modified DNA chemically by post-synthetic modification, and investigated their fluorescence properties and hybridization ability. The new fluorescent-labeled DNA bearing acridone will be useful as a DNA probe.
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