Structural property and enzymatic response of oxanine in DNA strands - DNA

Structural property and enzymatic response of oxanine in DNA strands
2006
Seung Pil Pack1,2, Akihiro Doi3, Mitsuru Nonogawa3, Nagendra Kumar Kamisetty3, Kamakshaiah Charyulu Devarayapalli3, Tsutomu Kodaki2,3 and Keisuke Makino1,2,3
1 Institute of Advanced Energy, Kyoto University, Gokasho, Uji 611-0011, Japan, 2 CREST, JST (Japan Science and Technology Agency), Kyoto, Japan, 3 International Innovation Center, Kyoto University, Yoshida-honmachi, Sakyo-ku, Kyoto 606-8501, Japan
Nucleic Acids Symposium Series 2006
Oxanine (Oxa, O), one of the major products generated from guanine (Gua) by nitrosative oxidation has been expected as mutagenic lesion involved in NO- or HNO2 -induced genotoxicity. Here, to elucidate the biological meaning of Oxa in DNA strands, several kinds of Oxa-containing oligodeoxynucleotides (Oxa-ODNs) were synthesized and applied to biophysical and biochemical characterization. CD and NMR analyses revealed that the conformations of all the Oxa-containing duplexes are basically B-type without causing any severe distortion in the whole DNA structure. It was also determined that restriction endonucleases recognize and cleave the specific base-sequence even when Gua was substituted by Oxa in the sequence. When Oxa-ODN was testified as substrates for other DNA-relevant enzymes, the enzymatic functions were not largely affected by Oxa.
Please visit the web site to view the article in its entirety.