Stabilisation by freeze-drying of cationically modified silica nanoparticles for gene delivery

Stabilisation by freeze-drying of cationically modified silica nanoparticles for gene delivery
M. Sametia, G. Bohra, M. N. V. Ravi Kumara, C. Kneuera, 1, U. Bakowskya, M. Nackenb, H. Schmidtb and C. -M. Lehr
6 November 2003
International Journal of Pharmaceutics
Abstract
Core shell silica particles with a hydrodynamic diameter of 28 nm, an IEP of 7.1 and a zeta potential of +35 mV at pH 4.0 were synthesised. The role of freeze-drying for the conservation of zwitterionic nanoparticles and the usefulness of different lyoprotective agents (LPA) for the minimisation of particle aggregation were studied. The activity of the nanoparticles was measured as DNA-binding capacity and transfection efficiency in Cos-1 cells before and after lyophilisation. It was found that massive aggregation occurred in the absence of LPA. Of the various LPAs screened in the present investigations, trehalose and glycerol were found to be well suited for conservation of cationically modified silica nanoparticles with simultaneous preservation of their DNA-binding and transfection activity in Cos-1 cells.