Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering

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Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering
Engineering Village 2
? 2006 Elsevier Inc
Accession number: 06249944079

Title: Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering

Authors: Luo, Jian; Leeman, Mats; Ballagi, Andras; Elfwing, Anders; Su, Zhiguo; Janson, Jan-Christer; Wahlund, Karl-Gustav

Author affiliation: Division of Technical Analytical Chemistry, Center for Chemistry and Chemical Engineering, Faculty of Engineering LTH, SE-221 00 Lund, Sweden

Serial title: Journal of Chromatography A

Abbreviated serial title: J. Chromatogr. A

Volume: v 1120

Issue: n 1-2

Monograph title: 29th International Symposium on High Performance Liquid Phase Separations and Related Techniques Part II

Issue date: Jul 7 2006

Publication year: 2006

Pages: p 158-164

Language: English

ISSN: 0021-9673

CODEN: JCRAEY

Document type: Journal article (JA)

Publisher: Elsevier, Amsterdam, 1000 AE, Netherlands

Abstract: The goal of this study was to investigate the applicability of asymmetrical flow field-flow fractionation-multi-angle light scattering (AsFlFFF-MALS) for size analysis of green fluorescent protein inclusion bodies (GFPIBs). The size distributions of GFPIBs prepared by various culture conditions were determined. For GFPIBs prepared at 37 ?C the peak maximum hydrodynamic diameter (dH) first increased and then decreased with the increase of the induction times in the presence of 0.1 and 2 mM isopropyl-?-d-thiogalactoside (IPTG). For GFPIBs prepared at 30 ?C the peak maximum dH was constant at about 700 nm irrespectively of the induction times and IPTG concentrations. ? 2005 Elsevier B.V. All rights reserved.

Number of references: 27

Ei main heading: Proteins

Ei controlled terms: Fluorescence - Light scattering - Fractionation - Hydrodynamics - Thermal effects - Concentration (process)

Uncontrolled terms: Asymmetrical flow field-flow fractionation - Multi-angle light scattering - Root mean square radius - Hydrodynamic diameter - Green fluorescence protein inclusion bodies

Ei classification codes: 804.1 Organic Compounds - 741.1 Light/Optics - 802.3 Chemical Operations - 531 Metallurgy and Metallography

Treatment: Experimental (EXP)

DOI: 10.1016/j.chroma.2005.11.048

Database: Compendex

Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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