Sequence-specific, electronic detection of oligonucleotides in blood, soil, and foodstuffs with the reagentless, reusable E-DNA sensor - Oligonucleotides

Sequence-specific, electronic detection of oligonucleotides in blood, soil, and foodstuffs with the reagentless, reusable E-DNA sensor
Engineering Village 2
2006 Elsevier Inc.
Accession number: 063510086917

Title: Sequence-specific, electronic detection of oligonucleotides in blood, soil, and foodstuffs with the reagentless, reusable E-DNA sensor

Authors: Lubin, Arica A.; Lai, Rebecca Y.; Baker, Brian R.; Heeger, Alan J.; Plaxco, Kevin W.

Author affiliation: Department of Chemistry and Biochemistry, Institute for Polymers and Organic Solids, University of California, Santa Barbara, CA 93106, United States

Serial title: Analytical Chemistry

Abbreviated serial title: Anal. Chem.

Volume: v 78

Issue: n 16

Issue date: Aug 15 2006

Publication year: 2006

Pages: p 5671-5677

Language: English

ISSN: 0003-2700

CODEN: ANCHAM

Document type: Journal article (JA)

Publisher: American Chemical Society, Columbus, OH 43210-3337, United States

Abstract: The ability to detect specific oligonucleotides in complex, contaminant-ridden samples, without the use of exogenous reagents and using a reusable, fully electronic platform could revolutionize the detection of pathogens in the clinic and in the field. Here, we characterize a label-free, electronic sensor, termed E-DNA, for its ability to simultaneously meet these challenging demands. We find that because signal generation is coupled to a hybridization-linked conformational change, rather than to only adsorption to the sensor surface, E-DNA is selective enough to detect oligonucleotides in complex, multicomponent samples, such as blood serum and soil. Moreover, E-DNA signaling is monotonically related to target complementarity, allowing the sensor to discriminate between mismatched targets: we readily detect the complementary 17-base target against a 50 000-fold excess of genomic DNA, can distinguish a three-base mismatch from perfect target directly in blood serum, and under ideal conditions, observe statistically significant differences between single-base mismatches. Finally, because the sensing components are linked to the electrode surface, E-DNA is reusable: a 30-s room temperature wash recovers >99% of the sensor signal. This work further supports the utility of E-DNA as a rapid, specific, and convenient method for the detection of DNA and RNA sequences. ? 2006 American Chemical Society.

Number of references: 32

Ei main heading: Blood

Ei controlled terms: Nucleic acids - Soils - DNA - Chemical sensors - Conformations - Adsorption - Electrodes

Uncontrolled terms: Contaminant-ridden samples - Electronic platform - Electronic sensor - Hybridization-linked conformational change

Ei classification codes: 461.2 Biological Materials - 801.2 Biochemistry - 804.1 Organic Compounds - 483.1 Soils & Soil Mechanics - 802.3 Chemical Operations - 704.1 Electric Components

Treatment: Theoretical (THR); Experimental (EXP)

DOI: 10.1021/ac0601819

Database: Compendex

Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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