STANDARD ITEM FOR FRACTIONAL DETERMINATION OF COMPONENT OF LIPOPROTEIN

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STANDARD ITEM FOR FRACTIONAL DETERMINATION OF COMPONENT OF LIPOPROTEIN
Publication Number: WO/2006/054519 International Application No.: PCT/JP2005/020845
Publication Date: 26.05.2006 International Filing Date: 14.11.2005

Int. Class.: G01N 33/96 (2006.01), G01N 33/92 (2006.01)
Applicants: KYOWA MEDEX CO., LTD. [JP/JP]; 8-10, Harumi 1-chome, Chuo-ku, Tokyo, 1046004 (JP) (All Except US).
BML, INC. [JP/JP]; 21-3, Sendagaya 5-chome, Shibuya-ku, Tokyo, 1510051 (JP) (All Except US).
TSUBOI, Isami [JP/JP]; c/o BML General Laboratory, 1361-1, Matoba, Kawagoe-shi, Saitama, 3501101 (JP) (US Only).
TAGUCHI, Michihiro [JP/JP]; c/o BML General Laboratory, 1361-1, Matoba, Kawagoe-shi, Saitama, 3501101 (JP) (US Only).
MIIKE, Akira [JP/JP]; c/o Head Office, KYOWA MEDEX CO., 8-10, Harumi 1-chome, Chuo-ku, Tokyo, 1046004 (JP) (US Only).
Inventors: TSUBOI, Isami [JP/JP]; c/o BML General Laboratory, 1361-1, Matoba, Kawagoe-shi, Saitama, 3501101 (JP).
TAGUCHI, Michihiro [JP/JP]; c/o BML General Laboratory, 1361-1, Matoba, Kawagoe-shi, Saitama, 3501101 (JP).
MIIKE, Akira [JP/JP]; c/o Head Office, KYOWA MEDEX CO., 8-10, Harumi 1-chome, Chuo-ku, Tokyo, 1046004 (JP).
Agent: HIROTA, Masanori; 3F Wakabayashi Bldg., 8-5, Akasaka 2-chome, Minato-ku, Tokyo, 1070052 (JP).
Priority Data: 2004-333770 17.11.2004 JP
Abstract: A lyophilized standard item for use in the fractional determination of a component to be measured of lipoprotein is produced by mixing a sulfonated buffering agent with a serum or plasma, lyophilizing the same and evaluating the content of component to be measured in the lyophilization product by the use of component to be measured whose quantity is known. An analytical curve is created by the use of this standard item and a reagent for fractional measurement of component to be measured contained in a lipoprotein. Any component to be measured of an analyte is measured with the use of the reagent for fractional measurement, and the concentration of component to be measured of the analyte is calculated from the thus obtained measurement value and the above analytical curve. Thus, there is provided a standard item for determination of a component to be measured of lipoprotein that ensures easy handling and, even after long-period storage, gives accurate measurement values, and provided a process for producing the standard item. Further, there is provided a method of fractional determination of a component to be measured of an analyte containing a lipoprotein, in which use is made of the above standard item.