SEGAL, a semi-automatic program for fitting sedimentation equilibrium patterns from analytical ultracentrifugation
SEGAL, a semi-automatic program for fitting sedimentation equilibrium patterns from analytical ultracentrifugation
Engineering Village 2
2006 Elsevier Inc.
Accession number: 9197958
Title: SEGAL, a semi-automatic program for fitting sedimentation equilibrium patterns from analytical ultracentrifugation
Authors: Machaidze, G.1 ; Lustig, A.1
Author affiliation: 1 M. E. Mueller Inst. of Struct. Biol., Basel Univ., Switzerland
Serial title: Journal of Biological Physics & Chemistry
Abbreviated serial title: J. Biol. Phys. Chem. (Switzerland)
Volume: 6
Issue: 2
Publication date: June 2006
Pages: 91-102
Language: English
ISSN: 1512-0856
Document type: Journal article (JA)
Publisher: Assoc. Modern Sci. Investigation and Collegium Basilea
Country of publication: Switzerland
Material Identity Number: P572-2006-004
Abstract: The paper describes a computer program (available for PC and Mac) for the evaluation of molecular weights from sedimentation equilibrium centrifuge runs using the Beckman XL-A/XL-I ultracentrifuge. The program can be used with both absorption and interference optical records, and requires no prior assumptions about the nature of the system under investigation (ideal, non-ideal, monodisperse, polydisperse, self-associating etc.). The user can simply choose and change the range of patterns for fitting to the data and simultaneously see the fitting result until the correlation is optimum. It is possible to produce fits to two parts of the same curve if more than one component is expected. The program can be used for the analysis of ultracentrifugation results for 2 and 6 sector centrifuge cells. The program supports the export of fitted data as text files, and can save the fitted images. The user-friendly interface, easy curve manipulation and simultaneous fitting make the program attractive for a wide range of users
Number of references: 12
Inspec controlled terms: biology computing - curve fitting - human computer interaction - molecular biophysics - molecular weight - proteins - user interfaces
Uncontrolled terms: SEGAL - semiautomatic program - sedimentation equilibrium pattern fitting - analytical ultracentrifugation - molecular weights - Beckman XL-A/XL-I ultracentrifuges - absorption optical records - interference optical records - user-friendly interface - curve fitting - molecular mass determination
Inspec classification codes: A8780 Biophysical instrumentation and techniques - A8715B Biomolecular structure, configuration, conformation, and active sites - A3620F Molecular weights of macromolecules - A0260 Numerical approximation and analysis - C7330 Biology and medical computing - C6180 User interfaces
Treatment: Experimental (EXP); Practical (PRA)
Discipline: Physics (A); Computers/Control engineering (C)
Database: Inspec
Copyright 2006, The Institution of Engineering and Technology
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