Reverse transcriptase assay kit, use thereof and method for analysis of RT activity in biological samples

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Reverse transcriptase assay kit, use thereof and method for analysis of RT activity in biological samples
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Publication number: US6849406

Patent number: 6849406

Patent authority: US

Kind: B1 - Utility Patent Grant (no pre-grant publication)

Title: Reverse transcriptase assay kit, use thereof and method for analysis of RT activity in biological samples

Inventors: K?llander, Clas (SE) ; Gronowitz, Simon (SE) ; Pettersson, Ingvar (SE)

Assignee: Cavidi Tech AB

Primary examiner: Winkler, Ulrike

Attorney, Agent or Firm: Bacon & Thomas

Publication date: 02/01/2005

Publication year: 2005

Application number: 926808

Unstandardized application number: 09926808

Filing date: 12/21/2001

Abstract: A reverse transcriptase (RT) assay kit for analysis of RT activity in biological samples is described. The kit comprises solid phase bound prA and/or pdA template(s) obtainable by contacting a polystyrene-based solid phase with a 1-methylimidazole-containing coupling solution, and RT-type adapted assay components selected from a buffer, divalent metal ion, chelator, polyamine, RNase inhibitor, reducing agent, salt, stabilizing agent, and detergent, and deoxynucleotide triphosphate, primer, protective agent and concentrated washing buffer, and optionally lyophilized reference enzyme(s), and further optionally lyophilized alkaline phosphatase conjugated anti-BrdU monoclonal antibody, alkaline phosphatase substrate buffer and alkaline phosphatase substrate, and written instructions for use of the assay kit. Further, a method and a use of the assay kit for the qualitative and quantitative analysis of RT activity in a biological sample, optionally followed by evaluation of the status of a RT activity related disorder or disease based on the result of the analysis of the RT activity, are disclosed.

Field of search: 435/5 - 435/6 - 435/7.1 - 435/91.1 - 435/91.2

Document type: US Grant

Priority information: SE 9902410 06/24/1999

IPC Code: C12Q1/68 SECTION C CHEMISTRY; METALLURGY
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS immunoassay ; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES 3
Measuring or testing processes involving enzymes or micro-organisms measuring or testing apparatus with condition measuring or sensing means, e.g. colony counters, ; Compositions therefor; Processes of preparing such compositions 3
involving nucleic acids 3

US Classification: 435/91.2 CHEMISTRY: MOLECULAR BIOLOGY AND MICROBIOLOGY
MICRO-ORGANISM, TISSUE CELL CULTURE OR ENZYME USING PROCESS TO SYNTHESIZE A DESIRED CHEMICAL COMPOUND OR COMPOSITION
Preparing compound containing saccharide radical
Preparing nitrogen-containing saccharide
N-glycoside
Nucleotide
Polynucleotide (e.g., nucleic acid, oligonucleotide, etc.)
Acellular exponential or geometric amplification (e.g., PCR, etc.)

435/91.1 CHEMISTRY: MOLECULAR BIOLOGY AND MICROBIOLOGY
MICRO-ORGANISM, TISSUE CELL CULTURE OR ENZYME USING PROCESS TO SYNTHESIZE A DESIRED CHEMICAL COMPOUND OR COMPOSITION
Preparing compound containing saccharide radical
Preparing nitrogen-containing saccharide
N-glycoside
Nucleotide
Polynucleotide (e.g., nucleic acid, oligonucleotide, etc.)

435/6 CHEMISTRY: MOLECULAR BIOLOGY AND MICROBIOLOGY
MEASURING OR TESTING PROCESS INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITION OR TEST STRIP THEREFORE; PROCESSES OF FORMING SUCH COMPOSITION OR TEST STRIP
Involving nucleic acid

435/5 CHEMISTRY: MOLECULAR BIOLOGY AND MICROBIOLOGY
MEASURING OR TESTING PROCESS INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITION OR TEST STRIP THEREFORE; PROCESSES OF FORMING SUCH COMPOSITION OR TEST STRIP
Involving virus or bacteriophage

Database: US Patents

Compilation and indexing terms, ? 2006 LexisNexis Univentio B.V.
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