Regulation of DNA duplex formation by hypoxic irradiation: one-electron reduction characteristics of oligodeoxynucleotides possessing 2-oxoalkyl functional group - DNA

Regulation of DNA duplex formation by hypoxic irradiation: one-electron reduction characteristics of oligodeoxynucleotides possessing 2-oxoalkyl functional group
2006
Kazuhito Tanabe, Hiroshi Kanezaki, Nao Hirata, Mitsuru Goto and Sei-ichi Nishimoto
Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Kyoto 615-8510, Japan
Nucleic Acids Symposium Series 2006
We synthesized oligodeoxynucleotides (ODN) possessing 2-oxoalkyl group on thymidine (doxoT) to characterize the radiolytic reduction in aqueous solution. Corresponding unmodified ODNs were generated from ODNs containing doxoT upon hypoxic irradiation. Enzymatic digestion of the duplex consisting of the hypoxically irradiated ODN containing doxoT and its complementary strand resulted in an efficient cleavage, but no cleavage was observed when a control duplex was digested without irradiation.
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