Reaction of atrazine with protein and its application to detection of protein
Reaction of atrazine with protein and its application to detection of protein
Engineering Village 2
? 2006 Elsevier Inc
Accession number: 06179840572
Title: Reaction of atrazine with protein and its application to detection of protein
Authors: Shi, Deng-Rong; Zhang, Tao; Ren, Li-Ping; Yu, Jin-Lian; Zhang, Chun-Rong; Rao, Zhen-Hong
Author affiliation: Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100094, China
Serial title: Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis
Abbreviated serial title: Guang Pu Xue Yu Guang Pu Fen Xi
Volume: v 26
Issue: n 3
Issue date: March 2006
Publication year: 2006
Pages: p 509-512
Language: Chinese
ISSN: 1000-0593
CODEN: GYGFED
Document type: Journal article (JA)
Publisher: Science Press, Beijing, China
Abstract: The resonance light scattering (RLS) technique and UV-Vis absorption spectra were applied to the investigation of the interaction between atrazine and bovine serum albumin (BSA). Under acidic conditions, the formation of atrazine-BSA supermolecule by Van der Waals force and N/O-H [midline ellipsis] p hydrogen bonds leads to a red shift of absorption band and strong RLS enhancement of atrazine. The characteristics and intensity of RLS were related to the pH, the concentration of atrazine, and temperature. Under the optimum conditions, the enhanced RLS intensities are in proportion to the concentration of BSA in the range of 0.05-100 ?g?mL-1. Based on the enhancement of the RLS, a simple and sensitive method for the determination of BSA was established. The detection limit (3s) is 12 ng?mL-1. Synthesis samples were determined with satisfactory results.
Number of references: 14
Database: Compendex
Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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