Rapid quantitative characterization of protein interactions by composition gradient static light scattering
Rapid quantitative characterization of protein interactions by composition gradient static light scattering
Engineering Village 2
? 2006 Elsevier Inc
Accession number: 8915527
Title: Rapid quantitative characterization of protein interactions by composition gradient static light scattering
Authors: Kameyama, K.1 ; Minton, A.P.1
Author affiliation: 1 Laboratory of Biochem. & Genetics, Nat. Inst. of Health, Bethesda, MD, USA
Serial title: Biophysical Journal
Abbreviated serial title: Biophys. J. (USA)
Volume: 90
Issue: 6
Publication date: 15 March 2006
Pages: 2164-9
Language: English
ISSN: 0006-3495
CODEN: BIOJAU
Document type: Journal article (JA)
Publisher: Biophys. Soc
Country of publication: USA
Material Identity Number: B154-2006-006
Abstract: Two new applications of the recently developed technique of composition gradient static light scattering (CG-SLS) are presented. 1), The method is demonstrated to be capable of detecting and quantitatively characterizing reversible association of chymotrypsin and bovine pancreatic trypsin inhibitor in a solution mixture and simultaneously occurring reversible self-association of chymotrypsin at low pH in the same mixture. The values of equilibrium constants for both self- and heteroassociation may be determined with reasonable precision from the analysis of data obtained from a single experiment requiring <15 min and <1 mg of each protein. 2), Analysis of the results of a single CG-SLS experiment carried out on Ftsz, a protein that self-associates to form linear oligomers of indefinite size in the presence of guanosine diphosphate, yields the dependence of the equilibrium constant for monomer addition upon oligomer size
Number of references: 14
Inspec controlled terms: association - bio-optics - biochemistry - biological techniques - light scattering - molecular biophysics - pH - proteins
Uncontrolled terms: protein interactions - composition gradient static light scattering - reversible association - chymotrypsin - bovine pancreatic trypsin inhibitor - reversible self-association - pH - equilibrium constants - Ftsz protein - linear oligomers - guanosine diphosphate
Inspec classification codes: A8780 Biophysical instrumentation and techniques - A8715D Physical chemistry of biomolecular solutions; condensed states - A8715M Interactions with radiations at the biomolecular level - A8750B Interactions of biosystems with radiations - A8230N Association, addition, and insertion - A8245 Electrochemistry and electrophoresis
Treatment: Practical (PRA); Experimental (EXP)
Discipline: Physics (A)
DOI: 10.1529/biophysj.105.074310
Database: Inspec
Copyright 2006, The Institution of Engineering and Technology
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