Preparation of diverse metal binding sites along the helical structure of duplex DNA - DNA

Preparation of diverse metal binding sites along the helical structure of duplex DNA
2006
Kumiko Sugiyama and Akira Ono
Department of Material and Life Chemistry, Faculty of Engineering, Kanagawa University, 3-27-1 Rokkakubashi, Kanagawa-ku, Yokohama, Kanagawa 221-8686, Japan
Nucleic Acids Symposium Series 2006
DNA duplexes containing 4-N-carboxymethylcytosine (1) were synthesized: duplex-I (5'-GTGACCA1TGCAG TG-3':3'-CACTGGT1ACGTCAC-5'), duplex-II (5'-GC CCA1TCCA1TGCG-3':3'-CG GGT1AGGT1ACGC-5'), and duplex-III (5'-GCTCCA11TGCACCG-3':3'-CGAG GT11ACGTGGC-5'). In the presence of Cu(II) or Ni(II) ions, duplex-I and duplex-II, which contain isolated 1-1 pair(s), were stabilized. In contrast, duplex-III, which contained consecutive 1-1 pairs, was stabilized significantly in the presence of Cu(II) ions, while the addition of Ni(II) ions had no notable effect on its thermal denaturation profile.
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