Polyamine Deactivation of Integrated Poly(dimethylsiloxane) Structures Investigated by Radionuclide Imaging and Capillary Electrophoresis Experiments - Analytical Methods & Tools for Lyophilization

Polyamine Deactivation of Integrated Poly(dimethylsiloxane) Structures Investigated by Radionuclide Imaging and Capillary Electrophoresis Experiments
Received for review May 20, 2004. Accepted November 2, 2004.
Web Release Date: December 30, 2004
Sara K. Bergstr?…¨?m, Niklas Edenwall, Martin Lav?…¨©n, Irina Velikyan, Bengt L?…¨ïngstr?…¨?m, and Karin E. Markides*
Anal. Chem.
ACS Publications
Copyright © 2006 American Chemical Society
Abstract:
The poly(dimethylsiloxane) (PDMS) material provides a number of advantageous features, such as flexibility, elasticity, and transparency, making it useful in integrated analytical systems. Hard fused-silica capillary structures and soft PDMS channels can easily be combined by a tight fit, which offers many alternatives for structure combinations. PDMS and fused silica are in different ways prone to adsorption of low levels of organic compounds. The need for modification of the inner wall surface of PDMS channels may often be necessary, and in this paper, we describe an easy and effective method using the amine-containing polymer PolyE-323 to deactivate both fused-silica and PDMS surfaces. The adsorption of selected peptides to untreated surfaces was compared to PolyE-323-modified surfaces, using both radionuclide imaging and capillary electrophoresis experiments. The polyamine modification displayed a substantially reduced adsorption of three hydrophobic test peptides compared to the native PDMS surface. Filling and storage of aqueous solution were also possible in PolyE-323-modified PDMS channels. In addition, hybrid microstructures of fused silica and PDMS could simultaneously be deactivated in one simple coating procedure.
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