Photo-induced proton gradients and ATP biosynthesis produced by vesicles encapsulated in a silica matrix

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Photo-induced proton gradients and ATP biosynthesis produced by vesicles encapsulated in a silica matrix
Engineering Village 2
2006 Elsevier Inc.
Accession number: 8546885

Title: Photo-induced proton gradients and ATP biosynthesis produced by vesicles encapsulated in a silica matrix

Authors: Luo, T.-J.M.1 ; Soong, R.; Lan, E.; Dunn, B.; Montemagno, C.

Author affiliation: 1 Dept. of Mater. Sci. & Eng., California Univ., Los Angeles, CA, USA

Serial title: Nature Materials

Abbreviated serial title: Nature Mater. (UK)

Volume: 4

Issue: 3

Publication date: March 2005

Pages: 220-4

Language: English

ISSN: 1476-1122

Document type: Journal article (JA)

Publisher: Nature Publishing Group

Country of publication: UK

Material Identity Number: O984-2005-003

Abstract: Sol-gel immobilization of soluble proteins has proven to be a viable method for stabilizing a wide variety of proteins in transparent inorganic matrices. The encapsulation of membrane-bound proteins has received much less attention, although work in this area suggests potential opportunities in microarray technology and high-throughput drug screening. The present paper describes a liposome/sol-gel architecture in which the liposome provides membrane structure and protein orientation to two transmembrane proteins, bacteriorhodopsin (bR) and F0F1-ATP synthase; the sol-gel encapsulation converts the liposomal solution into a robust material without compromising the intrinsic activity of the incorporated proteins. Here we report on two different proteoliposome-doped gels (proteogels) whose properties are determined by the transmembrane proteins. Proteogels containing bR proteoliposomes exhibit a stable proton gradient when irradiated with visible light, whereas proteogels containing proteoliposomes with both bR and F0F1-ATP synthase couple the photo-induced proton gradient to the production of ATP. These results demonstrate that materials based on the liposome/sol-gel architecture are able to harness the properties of transmembrane proteins and enable a variety of applications, from power generation and energy storage to the powering of molecular motors, and represent a new technology for performing complex chemical synthesis in a solid-state matrix

Number of references: 29

Inspec controlled terms: biochemistry - biomembranes - biomolecular effects of radiation - proteins - sol-gel processing - solubility

Uncontrolled terms: photo-induced proton gradients - ATP biosynthesis - vesicles - silica matrix - sol-gel encapsulation - sol-gel immobilization - soluble proteins - transparent inorganic matrices - membrane-bound proteins - microarray technology - high-throughput drug screening - liposome/sol-gel architecture - membrane structure - protein orientation - transmembrane proteins - bacteriorhodopsin - F0F1-ATP synthase - liposomal solution - intrinsic activity - proteoliposome-doped gels - proteogels - proteoliposomes - stable proton gradient - visible light irradiation - photo-induced proton gradient - power generation - energy storage - molecular motor power - complex chemical synthesis - solid-state matrix

Inspec classification codes: A8715D Physical chemistry of biomolecular solutions; condensed states - A8720E Natural and artificial biomembranes - A8715M Interactions with radiations at the biomolecular level - A8750B Interactions of biosystems with radiations

Treatment: Experimental (EXP)

Discipline: Physics (A)

DOI: 10.1038/nmat1322

Database: Inspec

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