PRESERVED, NON-INFECTIOUS CONTROL CELLS PREPARED BY THE MODULATION OR MODIFICATION OF NORMAL CELLS

12) Patent Application: (11) CA 2164131
ABSTRACT:
The invention describes the preparation of preserved, non-infectious control cells. The control cells are prepared by modification, activation or otherwise changing selected normal leukocyte cells, tissue culture cells or zenogenic transplants in immunosuppressed animals to have characteristics of abnormal cells or by the addition of cells from an established cell line, which has been similarly modified or activated, to a normal leukocyte population. The preferred method of preserving the control cells is by lyophilization using an isotonic 10% trehalose solution.
(72) Inventors (Country): MAPLES, JOHN A. (United States)
ROTH, PATRICIA A. (United States)
STIGLITZ, MELISSA J. (United States)
(73) Owners (Country): COULTER CORPORATION (United States)
(71) Applicants (Country):
(74) Agent: MACERA, JOHN STEPHEN
(45) Issued:
(86) PCT Filing Date: June 3 , 1994
(87) PCT Publication Date: Dec. 22, 1994
(51) International Class (IPC): C12N 5/00
G01N 15/12
A61K 35/18
G01N 33/554
G01N 33/96
Patent Cooperation Treaty (PCT): Yes (85) National Entry: Nov. 29, 1995
(86) PCT Filing number: PCT/US1994/006357
(87) International publication number: WO1994/029691
(30) Application priority data: Application No. Country Date
08/073,937 United States June 8 , 1993
Availability of licence: N/A
Language of filing: English