On-line preconcentration of protein in capillary electrophoresis with an end-column cellulose acetate-based porous membrane
On-line preconcentration of protein in capillary electrophoresis with an end-column cellulose acetate-based porous membrane
Engineering Village 2
? 2006 Elsevier Inc
Accession number: 06209878347
Title: On-line preconcentration of protein in capillary electrophoresis with an end-column cellulose acetate-based porous membrane
Authors: Yang, Bingcheng; Zhang, Feifang; Tian, Hongzhe; Guan, Yafeng
Author affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China
Serial title: Journal of Chromatography A
Abbreviated serial title: J. Chromatogr. A
Volume: v 1117
Issue: n 2
Issue date: Jun 9 2006
Publication year: 2006
Pages: p 214-218
Language: English
ISSN: 0021-9673
CODEN: JCRAEY
Document type: Journal article (JA)
Publisher: Elsevier, Amsterdam, 1000 AE, Netherlands
Abstract: A simple on-line preconcentration method of protein for capillary electrophoresis (CE) using a cellulose acetate (CA)-coated porous membrane was proposed. CA membrane is fabricated at one of the ends of the column that allows the passage of buffer ions but excludes larger protein molecules. Protein sample is continuously electrokinetically loaded and trapped by the membrane. When injection is completed, the direction of the electric field is switched and the trapped proteins are then separated by conventional CE procedure. The results achieved showed that the preconcentration mechanism of this method was based on size-exclusion effect. Bovine serum albumin (BSA) was used for model protein sample, and signal enhancement of 550-fold with 15 min injection time was achieved. ? 2006 Elsevier B.V. All rights reserved.
Number of references: 20
Ei main heading: Proteins
Ei controlled terms: Electrophoresis - Cellulose - Ions - Electric fields
Uncontrolled terms: Protein preconcentration - Capillary electrophoresis - Porous membranes
Ei classification codes: 804.1 Organic Compounds - 701.1 Electricity: Basic Concepts & Phenomena
Treatment: Theoretical (THR); Experimental (EXP)
DOI: 10.1016/j.chroma.2006.03.105
Database: Compendex
Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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