Novel adsorptive polyamine coating for enhanced capillary electrophoresis of basic proteins and peptides - Analytical Methods & Tools for Lyophilization

Novel adsorptive polyamine coating for enhanced capillary electrophoresis of basic proteins and peptides
Engineering Village 2
2006 Elsevier Inc.
Accession number: 06269972052

Title: Novel adsorptive polyamine coating for enhanced capillary electrophoresis of basic proteins and peptides

Authors: Puerta, Angel; Axen, Jakob; Soderberg, Lennart; Bergquist, Jonas

Author affiliation: Department of Physical and Analytical Chemistry, Analytical Chemistry, Biomedical Centre, SE-75124 Uppsala, Sweden

Serial title: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Abbreviated serial title: J. Chromatogr. B Anal. Technol. Biomed. Life Sci.

Volume: v 838

Issue: n 2

Issue date: Jul 11 2006

Publication year: 2006

Pages: p 113-121

Language: English

ISSN: 1570-0232

CODEN: JCBAAI

Document type: Journal article (JA)

Publisher: Elsevier, Amsterdam, 1000 AE, Netherlands

Abstract: In capillary electrophoresis (CE), the anionic and hydrophobic nature of the fused-silica capillary surface has long been known to present a problem in protein and peptide analysis. The use of capillary surface coating is one of the approaches to avoid the analyte-wall interactions. In this study, a new polymer, poly-LA 313, has been synthesized, physico-chemical characterized, and applied as polyamine coating for CE separations. The coating process is highly reproducible and provides fast separations of peptides and proteins in a few minutes and with high efficiency. The physically adsorbed polymer gives rise to a durable coating in the range of pH 2-10, in the presence of organic modifiers (acetonitrile and methanol) and with complex biological samples. The efficiency of the new cationic polymer was also tested performing protein and peptide separations with capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS). ? 2006 Elsevier B.V. All rights reserved.

Number of references: 41

Ei main heading: Proteins

Ei controlled terms: Electrophoresis - Amines - Organic coatings - Hydrophobicity - Polymers - Polypeptides - Ionization - Mass spectrometry

Uncontrolled terms: Capillary electrophoresis - Polyamine coating - Coated capillaries

Ei classification codes: 804.1 Organic Compounds - 701.1 Electricity: Basic Concepts & Phenomena - 801.3 Colloid Chemistry - 801.4.1 Electrochemistry - 813.2 Coating Materials - 931.2 Physical Properties of Gases, Liquids & Solids - 815.1 Polymeric Materials - 815.1.1 Organic Polymers - 802.2 Chemical Reactions

Treatment: Theoretical (THR); Experimental (EXP)

DOI: 10.1016/j.jchromb.2006.04.018

Database: Compendex

Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
Subscription required: http://www.engineeringvillage2.org