Lyophilization of Proteins - Market Research

Lyophilization of Proteins: Protein Purification Protocols
Second Edition
Ciar?n ?'F?g?in
December 2003
pps. 309-322
ISBN:
Series: Methods in Molecular Biology
Volume #: 244
Price: $30
Abstract:
Lyophilization, or freeze-drying, is a method for the preservation of labile materials in a dehydrated form. It can be particularly suitable for high-value biomolecules such as proteins. The process involves the removal of bulk water from a frozen protein solution by sublimation under vacuum with gentle heating (primary drying). This is followed by controlled heating to more elevated temperatures for removal of the remaining "bound" water from the protein preparation (secondary drying). Residual moisture levels are often lower than 1%. If the freeze-drying operation is carried out correctly, the protein will preserve all or most of its initial biological activity in the dry state. This dry state offers many advantages for long-term storage of the protein in question.
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Language: English