Lyophilisation and resuscitation of sporozoites of Theileria parva: preliminary experiments

Lyophilisation and resuscitation of sporozoites of Theileria parva: preliminary experiments
December 2003
T. Marcotty, D. Berkvens, R. K. Besa, B. Losson, T. T. Dolan, M. Madder, G. Chaka, P. Van den Bossche and J. Brandt
Vaccine, Volume 22, Issue 2, 12 December 2003, Pages 213-216
Science Direct
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Lyophilisation of Theileria parva sporozoite stabilates used for immunisation of cattle against East Coast fever would greatly improve vaccine storage and delivery. We report three attempts to lyophilise and resuscitate the sporozoites of T. parva. Sporozoites survived lyophilisation and were effective for immunisation. Lyophilised stabilate survived for 2 weeks at 5 ?C and for 12 weeks at -20 ?C. Although the viability of the stabilates was severely reduced during lyophilisation, this work suggests that this method has potential and should be considered for other Apicomplexan parasites such as Babesia sp. or Plasmodium sp.