Influence of temperature on the conformation of canine plasminogen: An analytical ultracentrifugation and dynamic light scattering study
Influence of temperature on the conformation of canine plasminogen: An analytical ultracentrifugation and dynamic light scattering study
Engineering Village 2
2006 Elsevier Inc
Accession number: 05409402245
Title: Influence of temperature on the conformation of canine plasminogen: An analytical ultracentrifugation and dynamic light scattering study
Authors: Kornblatt, Jack A.; Schuck, Peter
Author affiliation: Enzyme Research Group, Department of Biology, Concordia University, Montreal, Que. H4B 1R6, Canada
Serial title: Biochemistry
Abbreviated serial title: Biochemistry
Volume: v 44
Issue: n 39
Issue date: Oct 4 2005
Publication year: 2005
Pages: p 13122-13131
Language: English
ISSN: 0006-2960
CODEN: BICHAW
Document type: Journal article (JA)
Publisher: American Chemical Society, Columbus, OH 43210-3337, United States
Abstract: Plasminogen is known to undergo an extremely large conformational change when it binds ligands; the two well-established conformations are either closed (absence of external ligand) or open (presence of external ligand). We show here that plasminogen is more complicated than can be accommodated by a two-state, closed/open, model. Temperature changes induce large structural changes which can be detected with either dynamic light scattering or analytical ultracentrifugation. The temperature-induced changes are not related to the classical closed/open conformational change since both closed and open forms of the protein are similarly influenced. It appears as though the packing density of the protein increases as the temperature is raised. Over the range 4-20 ?C, the Stokes' radius of the classical closed plasminogen goes from 4.7 to 4.2 nm, and that of the classical open form goes from 5.55 to 5.0 nm. These changes in packing can be rationalized if temperature change induces a large conformational change and if this is accompanied by a large change in hydration, by a change in solute binding, or by a change in the total void volume of the protein. ? 2005 American Chemical Society.
Number of references: 43
Ei main heading: Animal cell culture
Ei controlled terms: Conformations - Temperature distribution - Light scattering - Binding energy
Uncontrolled terms: Canine plasminogen - Ligands - Ultracentrifugation
Ei classification codes: 461.2 Biological Materials - 801.4 Physical Chemistry - 641.1 Thermodynamics - 741.1 Light/Optics
Treatment: Experimental (EXP)
DOI: 10.1021/bi050895y
Database: Compendex
Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
This is a subscription site. You will need to subscribe to Engineering Village 2 to view the details.
Votes:13
Comments: 0