Increased sensitivity to chemotherapeutic drug by RNAi targeting Hsp701A in HepG2 cells

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Increased sensitivity to chemotherapeutic drug by RNAi targeting Hsp701A in HepG2 cells
September 2006
Zhu Q, Zhang WG, Wang LF, Wang F, Zhang Y, Yang AG
PubMed
AIM: To investigate the effects on chemotherapeutic sensitivity to the drugs of tumor cells induced by Hsp701A targeting siRNA expression vector. METHODS: Specific small interference RNA (siRNA) targeting Hsp701A gene was generated and its eukaryotic expression vector was constructed and transfected into human HepG2 cells.Sensitivity to the chemotherapeutic drug of HepG2 cells transfected with the vector was detected by MTT colorimetry. RESULTS: The eukaryotic expression vector of siRNA targeting Hsp701A was successfully constructed and transfected into human HepG2 cells. Sensitivity to the chemotherapeutic drug of HepG2 cells was increased after Hsp701A was knock down (P<0.05). CONCLUSION: Knockdown of Hsp701A gene by siRNA enhances sensitivity to chemotherapeutic drug in HepG2 cells.
NOTE: The actual article is written in Chinese.