In vitro study of lysozyme in poly(lactide-co-glycolide) microspheres with sucrose acetate isobutyrate - Protein

In vitro study of lysozyme in poly(lactide-co-glycolide) microspheres with sucrose acetate isobutyrate
Received 10 April 2006; revised 8 August 2006; accepted 14 August 2006. Available online 18 August 2006.
Eun Seong Leea, , , Min Jung Kwona, Hyeok Leea, Kun Nab and Jung Ju Kima
European Journal of Pharmaceutical Sciences
Volume 29, Issue 5 , December 2006
ScienceDirect
aPharmaceutical & Health Research Institute, Amore Pacific Corporation/R&D Center, 314-1, Bora-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-729, Republic of Korea
bDivision of Biotechnology, The Catholic University of Korea, 43-1 Yeokgok 2-dong, Wonmi-gu, Bucheon-si, Gyeonggi-do 420-743, Republic of Korea
Abstract
This study investigated the suitability of microsphere formulations for extended protein delivery and complete protein release. These microspheres were prepared by a multi-emulsion method and prepared using a mixture of poly(lactide-co-glycolide) (PLGA), RG 502H (lactide:glycolide = 50:50, MW 9300) and sucrose acetate isobutyrate (SAIB). SAIB embedded into the microspheres and mixed with PLGA, improved the efficiency of enzyme encapsulation. The in vitro release rate of lysozyme (Lys) from the microspheres was reduced due to the high viscosity of the added SAIB and less degradation of PLGA by SAIB. These properties enabled prolonged release of Lys for up to 2 months, characterized by a minimal initial burst of Lys and nearly zero-order protein release kinetics result from co-administration of sorbitan monooleate 80. When it is considered that degradation products of SAIB are inactive for labile proteins, SAIB may be regarded as a promising candidate for long-acting protein delivery.
Keywords: Protein delivery; Poly(lactide-co-glycolide); Sucrose acetate isobutyrate (SAIB); Polysaccharide; Lysozyme

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