In vitro selection of DNA aptamer against abrin toxin and aptamer-based abrin direct detection - Aptamers

In vitro selection of DNA aptamer against abrin toxin and aptamer-based abrin direct detection
2006 Oct 18
Tang J, Yu T, Guo L, Xie J, Shao N, He Z.
Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China
2006 Oct 18
Abrin toxin as the target protein, belongs to class II ribosome-inactivating proteins family, has high toxicity to eukaryotic cells. Here, we firstly report the DNA aptamers, isolated by in vitro selection, recognize abrin toxin with high affinity and specificity, and have the advantage of no cross-reaction with structure-similar protein ricin toxin over antibodies. Then, a highly selective and sensitive aptamer-based abrin assay was established using a molecular light switching reagent [Ru(phen)(2)(dppz)](2+) with a limit of detection of 1nM and a wide linear range from 1 to 400nM with the correlation coefficient of 0.993. This assay can be successfully directly performed not only in physiological buffer but also in more complicated biological matrix, such as diluted serum.
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