Immunoassay of P-glycoprotein on single cell by capillary electrophoresis with laser induced fluorescence detection
Immunoassay of P-glycoprotein on single cell by capillary electrophoresis with laser induced fluorescence detection
Engineering Village 2
2006 Elsevier Inc.
Accession number: 06029636690
Title: Immunoassay of P-glycoprotein on single cell by capillary electrophoresis with laser induced fluorescence detection
Authors: Xiao, Hua; Li, Xin; Zou, Hanfa; Yang, Ling; Yang, Yanqin; Wang, Yulin; Wang, Hailin; Le, X. Chris
Author affiliation: National Chromatographic R and A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
Serial title: Analytica Chimica Acta
Abbreviated serial title: Anal. Chim. Acta
Volume: v 556
Issue: n 2
Issue date: Jan 25 2006
Publication year: 2006
Pages: p 340-346
Language: English
ISSN: 0003-2670
CODEN: ACACAM
Document type: Journal article (JA)
Publisher: Elsevier, Amsterdam, 1000 AE, Netherlands
Abstract: The cellular mechanism based on P-glycoprotein (PGP) for its drug pump function has become very important in multidrug resistance (MDR) research. A method has been established to characterize PGP on single K562 cell by coupling capillary electrophoresis with laser induced fluorescence detection. A permeable intact cell after the immunoassay binding with fluorescence labeling antibody was injected into the capillary and directly separated without lysis. It was found that once 5-10 optional cells were detected in batch, the PGP amount on this cell line could be outlined and calculated clearly. The PGP amount on K562 MDR cell line is 3.88 times higher than that on K562 sensitive cell line. These two cell lines with immunoassay binding were also analyzed by injection of multi-cells in order to improve the throughput. A resistance factor so called multidrug resistance multiple (MRM) was introduced to evaluate the MDR difference between cell lines. The MRM values of the cell line K562 measured by single cell analysis are well correlated with those by flow cytometry, which also prove the validity of our method in single cell analysis for the possibility of cancer diagnosis, pharmacokinetics and drug screening in future. ? 2005 Elsevier B.V. All rights reserved.
Number of references: 33
Ei main heading: Proteins
Ei controlled terms: Cells - Immunology - Bioassay - Electrophoresis - Lasers - Fluorescence
Uncontrolled terms: Laser induced fluorescence detection - Capillary electrophoresis - P-glycoproteins - Resistance factor
Ei classification codes: 804.1 Organic Compounds - 461.2 Biological Materials - 461.9.1 Immunology - 461.6 Medicine - 701.1 Electricity: Basic Concepts & Phenomena - 801.3 Colloid Chemistry - 801.4.1 Electrochemistry - 744.1 Lasers, General - 741.1 Light/Optics
Treatment: Theoretical (THR)
DOI: 10.1016/j.aca.2005.09.051
Database: Compendex
Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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