Hollow Fiber Flow Field-Flow Fractionation of Proteins Using a Microbore Channel
Hollow Fiber Flow Field-Flow Fractionation of Proteins Using a Microbore Channel
Received for review February 17, 2005. Accepted April 20, 2005.
Web Release Date: May 18, 2005
Dukjin Kang and Myeong Hee Moon*
Anal. Chem.
ACS Publications
Copyright ? 2005 American Chemical Society
Department of Chemistry, Yonsei University, Seoul, 120-749, Korea
Abstract:
Protein separation through hollow fiber flow field-flow fractionation (HF FlFFF) at microflow rate regime was successfully achieved by employing a microbore hollow fiber. In most of the flow field-flow fractionation (FlFFF) techniques applied to the separation of proteins, including hollow fiber FlFFF (HF FlFFF), an outflow rate leading to a detector has typically been a few tenths of a milliliter per minute. In this study, it is demonstrated for the first time that 10 L/min outflow rate in HF FlFFF can be employed for a successful separation of proteins by utilizing a small inner diameter (450 m) hollow fiber. Initial evaluations of microbore HF FlFFF separation were made to improve separation efficiency by evaluating plate heights, sample recovery, and the limit of detection using protein standards. Microbore HF FlFFF was applied for the separation of low-abundance blood proteins depleted of high-abundance proteins from raw serum using immunoaffinity chromatography.
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