Heat Stability of the Lyophilized Sabin Poliovaccine - Lyophilization Articles & Reports

Heat Stability of the Lyophilized Sabin Poliovaccine
Received February 26, 2003. Accepted May 1, 2003
Hiroshi Shiomi, Tomoko Urasawa*1 and Shozo Urasawa*
Japanese Journal of Infectious Diseases
Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan
SUMMARY: In this study we lyophilized three types of live attenuated polioviruses (Sabin vaccine strains) and evaluated the lyophilized vaccine viruses' heat stability. The virus titers were measured after heating at 37?? and 45?? and then compared with the titers of conventional liquid vaccine viruses similarly treated. The results showed that lyophilization, while slightly reducing vaccine virus titers, had a far greater sparing effect on subsequent heat inactivation of lyophilized vaccine viruses, thus demonstrating its validity for the improvement of the vaccine.
*1Present address: Department of Liberal Arts and Sciences, Tenshi College, N13 E3, Higashi-ku, Sapporo 065-0013, Japan.
*Corresponding author: Mailing address: Faculty of Human Ecology, Fuji Women?s University, Minami 4-5, Hanakawa, Ishikari 061-3204, Japan. Tel: +81-133-74-3111, E-mail: urasawa@fujijoshi.ac.jp
You can view the abstract online. Full text is available in PDF format.