Extraction of squalene from yeast by supercritical carbon dioxide

Extraction of squalene from yeast by supercritical carbon dioxide
August 2003
P. Bhattacharjee1 and R.S. Singhal1
World Journal of Microbiology and Biotechnology
Issue: Volume 19, Number 6
SpringerLink
(1) Food and Fermentation Technology Department, University Institute of Chemical Technology, Matunga, Mumbai, 400 019, India
Abstract Squalene produced under anaerobic conditions, by a strain of Torulaspora delbrueckii was extracted from the biomass using supercritical carbon dioxide. Minimum use of solvent, lower time of isolation and a higher selectivity of extraction merit use of supercritical fluid extraction (SFE) technique over solvent extraction of squalene, as optimized and reported previously. A maximum squalene yield of 11.12 g g?1 (dry weight) of yeast cells was obtained at a temperature of 60 ?C and pressure of 250?255 bar at a constant flow rate of 0.2l min?1 of carbon dioxide. Lyophilization prior to SFE increased the squalene yield to 430.52 g g?1 dry weight of yeast cells, an amount that is far greater than that obtained by (2:1) chloroform?methanol solvent extraction.
Biomass - carbon dioxide - lipid extract - squalene - supercritical fluid extraction
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R.S. Singhal
Email: rekha@foodbio.udct.ernet.in
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