Evaluation of the xerovac process for the preparation of heat tolerant contagious bovine pleuropneumonia (CBPP) vaccine

Evaluation of the xerovac process for the preparation of heat tolerant contagious bovine pleuropneumonia (CBPP) vaccine
8 April 2005
Received 23 April 2004; accepted 10 November 2004. Available online 1 December 2004.
J.K. Litamoia, , , G. Ayeletb, and M.M. Rweyemamuc,
Vaccine
Volume 23, Issue 20 , 8 April 2005, Pages 2573-2579
ScienceDirect
aPANVAC, P.O. Box 1746, Debre Zeit, Ethiopia
bNational Veterinary Institute, P.O. Box 19, Debre-Zeit, Ethiopia
cAVIS College, London, UK
Abstract
The study was conducted with the aim of evaluating the xerovac process as a method for preparing contagious bovine pleuropneumonia (CBPP) vaccine with increased heat resistance. The thermo-protective effects of various concentrations of trehalose in mycoplasma growth medium, various concentrations of trehalose in the dehydration stabilizer and the importance of some divalent cations were assessed.
The results obtained indicate that a rapid dehydration of CBPP vaccine following the xerovac method and in an excipient composed of a high concentration of trehalose, renders the product more heat tolerant than a similar vaccine prepared using a regular or an extended freeze drying regime. It was also demonstrated that the addition of chitosan as a mycoplasma precipitating agent conferred additional heat resistance to the vaccine. It is suggested that the application of the xerovac process in the dehydration of CBPP vaccine offers the advantages of a faster, cheaper and easier process over the conventional dehydration methods like freeze drying.
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