Enhanced cellular uptake of a glutathione selective fluorogenic probe encapsulated in nanoparticles

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Enhanced cellular uptake of a glutathione selective fluorogenic probe encapsulated in nanoparticles
Engineering Village 2
2006 Elsevier Inc
Accession number: 06219898978

Title: Enhanced cellular uptake of a glutathione selective fluorogenic probe encapsulated in nanoparticles

Authors: Glewka, Eliza; Lamprecht, Alf; Ubrich, Nathalie; Maincent, Philippe; Lulek, Janina; Coulon, Joel; Leroy, Pierre

Author affiliation: Laboratoire de Pharmacie Galenique et Biopharmacie, UHP Nancy 1, Faculte de Pharmacie, F-54001 Nancy Cedex, France

Serial title: Nanotechnology

Abbreviated serial title: Nanotechnology

Volume: v 17

Issue: n 10

Issue date: May 28 2006

Publication year: 2006

Pages: p 2546-2552

Language: English

ISSN: 0957-4484

CODEN: NNOTER

Document type: Journal article (JA)

Publisher: Institute of Physics Publishing, Bristol, BS1 6BE, United Kingdom

Abstract: Selective fluorogenic probes for the labelling of intracellular reduced glutathione (GSH), i.e. ortho-phthaldialdehyde (OPA) and naphthalene-2,3- dicarboxaldehyde (NDA), have been encapsulated in polymeric nanoparticles (NPs) and the ability of the NPs to enhance uptake of the probe by microbial cells has been evaluated. Preparation of the probe-loaded NPs composed of Eudragit [registered trademark] E was based on an oil-in-water emulsification solvent evaporation method using an ultrasonic probe and polyvinyl alcohol as the surfactant. The encapsulation efficiency of the probes in lyophilized NPs was determined using high performance liquid chromatography (HPLC). A higher encapsulation rate of NDA than OPA was found: 47.6 ? 9.9 (n Combining double low line 6) and 2.1 ? 0.2% (n Combining double low line 3), respectively. The NDA-loaded particle diameter and zeta potential were 224.6 ? 14.7 nm and +40.9 ? 6.5 mV, respectively. After 20 min incubation of cultured Candida albicans yeast cells with either free NDA or NDA-loaded NPs (final NDA concentration 100 ?M), cells were harvested and corresponding lysates were analysed using HPLC coupled with spectrofluorimetric detection. Incubation of cells with NDA-loaded NPs increased intracellular levels of NDA-GSH adduct by about nine-fold in comparison with the free probe. Adhesion on the cells and the penetration behaviour of NPs loaded with either NDA or fluorescent label (Nile Red) were characterized qualitatively by confocal laser scanning microscopy. ? 2006 IOP Publishing Ltd.

Number of references: 27

Ei main heading: Nanostructured materials

Ei controlled terms: Encapsulation - Organic compounds - Polymers - Naphthalene - Emulsification - Evaporation - Polyvinyl alcohols - Yeast

Uncontrolled terms: Intracellular reduced glutathione (GSH), - Naphthalene-2,3- dicarboxaldehyde (NDA) - Microbial cells - Encapsulation efficiency

Ei classification codes: 933.1 Crystalline Solids - 714.2 Semiconductor Devices & Integrated Circuits - 804.1 Organic Compounds - 815.1 Polymeric Materials - 802.3 Chemical Operations - 815.1.1 Organic Polymers - 822.3 Food Products

Treatment: Theoretical (THR); Experimental (EXP)

DOI: 10.1088/0957-4484/17/10/018

Database: Compendex

Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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