Electrochemical detection of DNA single base mismatch by the use of strand exchange reaction - DNA

Electrochemical detection of DNA single base mismatch by the use of strand exchange reaction
2006
Satoshi Kumamoto1,3, Atsushi Maruyama2,3, Mitsunobu Nakamura1 and Kazushige Yamana1,3
1 Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, 2167 Syosya, Himeji 671-2201, Japan, 2 Institute for Materials Chemistry and Engineering, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan, 3 CREST of Japan Science and Technology Agency, Japan
Nucleic Acids Symposium Series 2006
The electrochemical method based on the strand exchange reaction (SER) for the detection of DNA single-base mismatch has been developed. Different electrochemical responses due to the slower SER rates for mismatch containing DNA than fully matched DNA were observed by using the redox-modified partial duplex DNA immobilized on gold electrode as an analytical probe.
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