Effect of various growth media upon survival during storage of freeze-dried Enterococcus faecalis and Enterococcus durans

Effect of various growth media upon survival during storage of freeze-dried Enterococcus faecalis and Enterococcus durans
June 2003
A.S. Carvalho1, J. Silva1, P. Ho1,2, P. Teixeira1, F.X. Malcata1 and P. Gibbs1
Journal of Applied Microbiology
Volume 94 Issue 6 Page 947
Blackwell Synergy
1Escola Superior de Biotecnologia, Universidade Cat?lica Portuguesa, Porto, Portugal, 2Departamento de Ci?ncias de Engenharia e Tecnologia, Escola Superior de Tecnologia e Gest?o, Instituto Polit?cnico de Viana do Castelo, Viana do Castelo, Portugal
Abstract
Aims: The effects of three different growth media (MRS, M17 and Lee's) on survival during freeze-drying and subsequent storage of six strains of Enterococcus faecalis and two strains of E. durans were investigated.
Methods and Results: Distinct Enterococcus spp. strains were grown on M17, MRS and Lee's broth, freeze-dried and stored at 20?C in air under darkness. At regular intervals throughout storage, freeze-dried samples were rehydrated and then plated on M17 agar.
Conclusions: A higher survival rate during storage of dried E. durans was obtained when growth occurred in MRS. The same effect was not observed, however, for the majority of E. faecalis strains, which clearly survived better in the dried state when this organism had been grown in M17 or Lee's medium.
Significance and Impact of Study: The survival of the dried Enterococcus spp. tested during storage was shown to be strain-specific and dependent on the growth medium.
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