Direct comparison of capillary electrophoresis and capillary liquid chromatography hyphenated to collision-cell inductively coupled plasma mass spectrometry for the investigation of Cd-, Cu- and Zn-containing metalloproteins
Direct comparison of capillary electrophoresis and capillary liquid chromatography hyphenated to collision-cell inductively coupled plasma mass spectrometry for the investigation of Cd-, Cu- and Zn-containing metalloproteins
Engineering Village 2
2006 Elsevier Inc.
Accession number: 06209880141
Title: Direct comparison of capillary electrophoresis and capillary liquid chromatography hyphenated to collision-cell inductively coupled plasma mass spectrometry for the investigation of Cd-, Cu- and Zn-containing metalloproteins
Authors: Montes-Bayon, Maria; Profrock, Daniel; Sanz-Medel, Alfredo; Prange, Andreas
Author affiliation: University of Oviedo, Department of Physical and Analytical Chemistry, 33006 Oviedo, Spain
Serial title: Journal of Chromatography A
Abbreviated serial title: J. Chromatogr. A
Volume: v 1114
Issue: n 1
Issue date: May 5 2006
Publication year: 2006
Pages: p 138-144
Language: English
ISSN: 0021-9673
CODEN: JCRAEY
Document type: Journal article (JA)
Publisher: Elsevier, Amsterdam, 1000 AE, Netherlands
Abstract: Capillary liquid chromatography (cLC) and capillary electrophoresis (CE) have been critically compared for the separation of metalloproteins when using collision-cell inductively coupled plasma mass spectrometry (ICP-CC-MS) as detection system. For cLC separation, the selected column was a C8 (0.3 mm I.D.) and the separation conditions involved a gradient up to 80% methanol in 10 mM ammonium acetate buffer (pH 7.4). The low flow rate used (3 ?L min-1) permitted the utilization of a high methanol content maintaining the sensitivity along the whole chromatographic run. For this purpose, a new low-flow interface has been developed based on a total consumption nebulizer. Similarly, CE has been studied as separation technique using a 75 ?m I.D. fused silica capillary and a running buffer of 20 mM Tris-HNO3 (pH 7.4) and working at 30 kV. Metallothionein (mixture of MT-I and -II) and superoxide dismutase (SOD) have been used as protein models in order to evaluate the separation/detection capabilities using the same injection volumes in both systems (20 nL). For both hybrid systems, separation parameters such as retention factor, numbers of theoretical plates, tailing factor and resolution have been critically compared. Also, the analytical performance characteristics of both hybrid systems have been evaluated and tested by analyzing the Cu-, Zn-species present in red blood cell extracts in order to explore more adequate separation methodology for the analysis of metalloproteins in complex matrices. ? 2006 Elsevier B.V. All rights reserved.
Number of references: 22
Ei main heading: Proteins
Ei controlled terms: Zinc - Copper - Carbon - Mass spectrometry - Electrophoresis - Liquid chromatography
Uncontrolled terms: cLC - Collision cell-reaction cell ICP-MS - Metalloproteins - Capillary electrophoresis (CE)
Ei classification codes: 804.1 Organic Compounds - 546.3 Zinc & Alloys - 544.1 Copper - 801 Chemistry - 701.1 Electricity: Basic Concepts & Phenomena - 801.3 Colloid Chemistry - 801.4.1 Electrochemistry
Treatment: Theoretical (THR)
DOI: 10.1016/j.chroma.2006.02.028
Database: Compendex
Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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