Determination of proteins at nanogram levels with Bordeaux red based on the enhancement of resonance light scattering
Determination of proteins at nanogram levels with Bordeaux red based on the enhancement of resonance light scattering
Engineering Village 2
? 2006 Elsevier Inc
Accession number: 06199867617
Title: Determination of proteins at nanogram levels with Bordeaux red based on the enhancement of resonance light scattering
Authors: Feng, Suling; Pan, Zihong; Fan, Jing
Author affiliation: College of Chemical and Environmental Sciences, Henan Normal University, Henan Xinxiang, 453007, China
Serial title: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Abbreviated serial title: Spectrochim. Acta Part A Mol. Biomol. Spectrosc.
Volume: v 64
Issue: n 3
Issue date: June 2006
Publication year: 2006
Pages: p 574-579
Language: English
ISSN: 1386-1425
CODEN: SAMCAS
Document type: Journal article (JA)
Publisher: Elsevier, Amsterdam, 1000 AE, Netherlands
Abstract: A simple, sensitive and selective method was proposed for the determination of proteins by using a resonance light scattering technique. The weak resonance light scattering (RLS) of Bordeaux red (BR) can be enhanced greatly in the pH range 3.87-3.96 by the addition of micro amounts of proteins, resulting in four characteristic peaks in the wavelength range 250-600 nm. At the maximal wavelength of 363 nm, the enhanced RLS is proportional to the concentration of proteins in the range 0.12-10.8 ?g ml-1 for bovine serum albumin (BSA) and 0.24-18.0 ?g ml-1 for human serum albumin (HSA). The detection limits were 40.0 and 52.9 ng ml-1 for BSA and HSA, respectively. The present method has been applied to the determination of total proteins in human serum, urine and saliva samples. The obtained results are in good agreement with those obtained by the Bradford method with relative standard deviations (R.S.D.) of 0.9-2.3%. ? 2005 Elsevier B.V. All rights reserved.
Number of references: 30
Ei main heading: Proteins
Ei controlled terms: Nanostructured materials - Light scattering - Concentration (process) - Statistical methods
Uncontrolled terms: Bordeaux red - Resonance light scattering - Protein determination
Ei classification codes: 804.1 Organic Compounds - 933.1 Crystalline Solids - 741.1 Light/Optics - 802.3 Chemical Operations - 922.2 Mathematical Statistics
Treatment: Theoretical (THR); Experimental (EXP)
DOI: 10.1016/j.saa.2005.07.056
Database: Compendex
Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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