Delivery of antibody-captured proteins into living cells using PTD-fused protein A

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Delivery of antibody-captured proteins into living cells using PTD-fused protein A
June 28, 2006
Masayasu Mie, Kazuto Mori, Hisakage Funabashi and Eiry Kobatake
Springer
Abstract Protein transduction domain (PTD)-mediated protein delivery into animal cells is a useful technique for regulating cellular functions. Proteins captured by antibodies were delivered into living cells using an antibody/PTD-fused protein A complex. As a model protein, fluorescent-modified antibodies, captured by their respective primary antibody, were analyzed by fluorescence-activated cell sorting (FACS) which showed that the fluorescent-modified antibodies were directly delivered into cells. Peroxidase, captured by its specific antibody, was also delivered into cells and retained its activity.
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