Construction of plasmids for RNA interference and in vitro transcription of double-stranded RNA

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Construction of plasmids for RNA interference and in vitro transcription of double-stranded RNA
2006
Timmons L.
Methods Mol Biol.
Double-stranded RNA (dsRNA)-induced gene silencing in Caenorhabditis elegans involves the manufacture and delivery of defined sequences of dsRNA to the organism, followed by a careful monitoring for loss-of-function phenocopies in treated animals. In this chapter, we describe how to generate DNAs that can be used as templates for transcription of dsRNA.
PMID: 16988429 [PubMed - in process]