Conjugation of ?-Fe2O3 nanoparticles with single strand oligonucleotides - Oligonucleotides

Conjugation of ?-Fe2O3 nanoparticles with single strand oligonucleotides
Available online 3 March 2006.
C.W. Leea, K.T. Huangb, P.K. Weia and Y.D. Yaob, ,
Journal of Magnetism and Magnetic Materials
Volume 304, Issue 1 , September 2006
ScienceDirect
aResearch Center for Applied Science, Academia Sinica, Taipei 115, Taiwan
bInstitute of Physics, Academia Sinica, Taipei 115, Taiwan
Abstract
Following the thermodecomposition synthetic route, water soluble ?-Fe2O3 nanoparticles have been successfully prepared, with average size 8.8?1.3 nm. Based on the process we developed, the magnetic particles have carboxyl group on their surfaces. By using 1-ethyl-3-(3-dimrthylaminopropyl)carbodiimide hydrochloride [EDC] as a liker reagent, we successfully modified a protein, streptavidin, on the surface of ?-Fe2O3 nanoparticles. With the strong affinity between biotin with streptavidin, we could use the streptavidin-modified magnetic nanoparticles to separate the rare biotin functionalized molecules from the mixed solution. On the other hand, streptavidin functionalized Fe2O3 can catch a biotin labeled single strand oligonucleotides through the strong affinity between streptavidin and biotin. The oligonucleotides functionalized magnetic nanoparticle would separate a specific oligonucleotide from a mixture. This design may be used to create a technique to detect diseases in the future.
Keywords: Nanoparticle; Oligonucleotide; Protein

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