Characterization of lignin using multi-angle laser light scattering and atomic force microscopy
Characterization of lignin using multi-angle laser light scattering and atomic force microscopy
Engineering Village 2
2006 Elsevier Inc.
Accession number: 05529619810
Title: Characterization of lignin using multi-angle laser light scattering and atomic force microscopy
Authors: Gidh, Aarti V.; Decker, Stephen R.; See, Chun H.; Himmel, Michael E.; Williford, Clint W.
Author affiliation: National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO 80401, United States
Serial title: Analytica Chimica Acta
Abbreviated serial title: Anal. Chim. Acta
Volume: v 555
Issue: n 2
Issue date: Jan 12 2006
Publication year: 2006
Pages: p 250-258
Language: English
ISSN: 0003-2670
CODEN: ACACAM
Document type: Journal article (JA)
Publisher: Elsevier, Amsterdam, 1000 AE, Netherlands
Abstract: Small differences in the isolation techniques of lignin can result in significant changes in its molecular structure and configuration. Light scattering (evaluated at 18 different angles in a plane), Atomic Force Microscopy (AFM) and Near Infrared Spectroscopy (NIR) proved very effective for evaluating the characteristics of lignin. Zimm plots were generated using Zimm, Debye and Berry formalisms to evaluate the weight average molecular weight (MW), radius of gyration (rg), hydrodynamic radius (rh) and second virial coefficient (A2). Two types of lignin and nine different solvents were used for the study, to analyze the conformation of lignin molecules in different solvents expected to be used in lignin degradation and subsequent analysis. Absolute MW and rg decreased and the dn/dc increased when the solvent used for lignin was changed from water to sodium hydroxide. The two types of lignin also exhibited different values for all the above estimated parameters. This study also highlighted the differences between the unlyophilized and lyophilized lignin in terms of aggregation, pH dependence and stability over time. This aggregation has never been seen on a ultraviolet (UV) or refractive index (RI) detector that has been used so far for liquid chromatography (LC) reducing the reliability of lignin depolymerization data obtained without light scattering. ? 2005 Elsevier B.V. All rights reserved.
Number of references: 29
Ei main heading: Proteins
Ei controlled terms: Characterization - Light scattering - Atomic force microscopy - Microstructure - Molecular weight - Solvents - Refractive index - Agglomeration - Depolymerization
Uncontrolled terms: Molecular conformation - Multi-angle laser light scattering - Virial coefficient - Near Infrared Spectroscopy (NIR)
Ei classification codes: 804.1 Organic Compounds - 741.1 Light/Optics - 741.3 Optical Devices & Systems - 531.2 Metallography - 931.3 Atomic & Molecular Physics - 803 Chemical Agents and Basic Industrial Chemicals - 802.3 Chemical Operations - 815.2 Polymerization
Treatment: Theoretical (THR); Experimental (EXP)
DOI: 10.1016/j.aca.2005.09.023
Database: Compendex
Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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