Cationic starch derivatives as dynamic coating additives for protein analysis in capillary electrophoresis
Cationic starch derivatives as dynamic coating additives for protein analysis in capillary electrophoresis
Engineering Village 2
? 2006 Elsevier Inc
Accession number: 06139793983
Title: Cationic starch derivatives as dynamic coating additives for protein analysis in capillary electrophoresis
Authors: Sakai-Kato, Kumiko; Kato, Masaru; Nakajima, Tohru; Toyo'oka, Toshimasa; Imai, Kazuhiro; Utsunomiya-Tate, Naoko
Author affiliation: Research Institute of Pharmaceutical Sciences, Musashino University, Nishitokyo-shi, Tokyo 202-8585, Japan
Serial title: Journal of Chromatography A
Abbreviated serial title: J. Chromatogr. A
Volume: v 1111
Issue: n 2
Issue date: Apr 14 2006
Publication year: 2006
Pages: p 127-132
Language: English
ISSN: 0021-9673
CODEN: JCRAEY
Document type: Journal article (JA)
Publisher: Elsevier, Amsterdam, 1000 AE, Netherlands
Abstract: Positively charged starch derivatives were used to modify the inner surface of fused-silica capillaries by addition to running buffer, which were subsequently employed in capillary electrophoresis (CE). Capillaries coated with the cationic starch derivatives were shown to generate a stable, reversed electroosmotic flow (EOF) in the investigated pH range of 3-9. The presented coating procedure was fast, based on a simple rinsing protocol where the polymer created a physically adsorbed, cationic polymer layer. Among the additives studied, a quaternary ammonium starch derivative showed a fast EOF mobility and effectively suppressed the adsorption of proteins. The intra- and inter-day reproducibility of the coating referring to the EOF mobility were satisfactory with relative standard deviation (RSD) of 0.27 and 1.67%, respectively. The coating enabled separation of some protein mixtures including basic proteins within l3 min with efficiencies up to 280,000 plates/m. In addition, this cationic starch derivative possessed a good solubility (about 100 mg/mL), and it does not significantly contribute to the background adsorption in the UV region of 190-400 nm. ? 2005 Elsevier B.V. All rights reserved.
Number of references: 34
Ei main heading: Starch
Ei controlled terms: Coatings - Additives - Proteins - Electrophoresis - Fused silica - pH effects
Uncontrolled terms: Capillary electrophoresis - Dynamic coating - Cationic starch derivatives - Protein separation
Ei classification codes: 804.1 Organic Compounds - 815.1.1 Organic Polymers - 813.2 Coating Materials - 803 Chemical Agents and Basic Industrial Chemicals - 701.1 Electricity: Basic Concepts & Phenomena - 801.3 Colloid Chemistry - 801.4.1 Electrochemistry - 812.3 Glass - 801.1 Chemistry, General
Treatment: Theoretical (THR); Experimental (EXP)
DOI: 10.1016/j.chroma.2005.06.097
Database: Compendex
Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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