Capillary electrophoretic separation and fractionation of hydrophobic peptides onto a pre-structured matrix assisted laser desorption/ionization target for mass spectrometric analysis

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Capillary electrophoretic separation and fractionation of hydrophobic peptides onto a pre-structured matrix assisted laser desorption/ionization target for mass spectrometric analysis
Engineering Village 2
? 2006 Elsevier Inc
Accession number: 06109737818

Title: Capillary electrophoretic separation and fractionation of hydrophobic peptides onto a pre-structured matrix assisted laser desorption/ionization target for mass spectrometric analysis

Authors: Jacksen, Johan; Redeby, Theres; Emmer, Asa

Author affiliation: Department of Chemistry, Division of Analytical Chemistry, Royal Institute of Technology (KTH), SE-100 44 Stockholm, Sweden

Serial title: Journal of Separation Science

Abbreviated serial title: J. Sep. Sci.

Volume: v 29

Issue: n 2

Issue date: February 2006

Publication year: 2006

Pages: p 288-295

Language: English

ISSN: 1615-9306

CODEN: JSSCCJ

Document type: Journal article (JA)

Publisher: Wiley-VCH Verlag, Weinheim, D-69451, Germany

Abstract: A CE separation of hydrophobic peptides followed by fractionation onto a prestructured MALDI target and off-line MS analysis was performed. An improved and partially automated manufacturing procedure of the previously described MALDI target is presented. This target is structurally coated with silicone and especially developed for hydrophobic peptides and proteins. Here, the target plate was designed specifically for the CE fraction collection. Different solvents were evaluated to meet the requirements of peptide solubility and compatibility to both the CE and MALDI methods and to the fractionation procedure. CE-MALDI-MS analysis of nine highly hydrophobic peptides from cyanogen bromide-digested bacteriorhodopsin is demonstrated. ? 2006 Wiley-VCH Verlag GmbH and Co. KGaA, Weinheim.

Number of references: 42

Ei main heading: Polypeptides

Ei controlled terms: Hydrophobicity - Separation - Electrophoresis - Fractionation - Desorption - Ionization - Mass spectrometry

Uncontrolled terms: Hydrophobic peptides - Capillary electrophoresis - Matrix-assisted laser desorption - Ionization mass spectrometry - Prestructured target

Ei classification codes: 804.1 Organic Compounds - 931.2 Physical Properties of Gases, Liquids & Solids - 802.3 Chemical Operations - 801.4.1 Electrochemistry - 802.2 Chemical Reactions - 801.1 Chemistry, General

Treatment: Theoretical (THR)

DOI: 10.1002/jssc.200500338

Database: Compendex

Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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