Association complexes between ovalbumin and cyclodextrins have no effect on the immunological properties of ovalbumin - Analytical Methods & Tools for Lyophilization

Research paper
Association complexes between ovalbumin and cyclodextrins have no effect on the immunological properties of ovalbumin
Received 28 May 2003; Revised 31 October 2003; accepted 31 October 2003. Available online 9 January 2004.
A. O. Kamphorst a, I. Mendes de S? b, A. M. C. Faria a and R. D. Sinisterra , , b
European Journal of Pharmaceutics and Biopharmaceutics
Volume 57, Issue 2 , March 2004
ScienceDirect
Abstract
Delivery systems are designed to deliver necessary amounts of drugs without modifying their biological features. Cyclodextrins (CD) are potential candidates for such a role due to their ability to alter physical and chemical properties of guest molecules by the formation of inclusion/association complexes. They have already been used to stabilize and solubilize peptides and proteins of biological importance. However, no systematic study has been reported on their immunological effects upon coupling to such proteins. Herein, we prepared and characterized the association complexes between a-, ?- and hydroxypropyl-?-cyclodextrin and ovalbumin (Ova). Afterward we tested the effect of CD coupling in the Ova antigenicity and the immunological effects of CD coupling on Ova oral and subcutaneous administration. Our results clearly show that CD-coupled Ova elicits the same immunological activities as uncoupled Ova. Therefore, we conclude that CDs are immunologically safe for use as delivery systems in animals.
Author Keywords: Author Keywords: Cyclodextrin; Ovalbumin; Immunization; Oral tolerance; Antigenicity
Ova, ovalbumin; CFA, complete Freund's adjuvant; ELISA, enzyme-linked immunosorbent assay; i.p., intraperitoneal; s.c., subcutaneous; CD, cyclodextrins; FTIR, Fourier transform infrared spectroscopy; TG, thermogravimetry curve; DTG, first derivative of thermogravimetry curve; DSC, differential scanning calorimetry; XRD, X-ray pattern diffraction

Corresponding author. Departamento de QuÃmica, Instituto de CiÕncias Exatas, Universidade Federal de Minas Gerais (UFMG), Av. AntŸnio Carlos, 6627 Pampulha, Belo Horizonte, MG 31270-901, Brazil. Tel.: +55-31-3499-5778; fax: +55-31-3499-5700
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