Arginine-rich intracellular delivery peptides noncovalently transport protein into living cells - Protein

Arginine-rich intracellular delivery peptides noncovalently transport protein into living cells
4 August 2006
Ya-Hui Wanga, Chung-Pin Chena, Ming-Huan Chanb, Microsugar Changa, Yu-Wun Houa, Hwei-Hsien Chenb, Hui-Ru Hsub, Kevin Liua and Han-Jung Lee
Biochemical and Biophysical Research Communications
Abstract
Plasma membranes of plant or animal cells are generally impermeable to peptides or proteins. Many basic peptides have previously been investigated and covalently cross-linked with cargoes for cellular internalization. In the current study, we demonstrate that arginine-rich intracellular delivery (AID) peptides are able to deliver fluorescent proteins or ?-galactosidase enzyme into animal and plant cells, as well as animal tissue. Cellular internalization and transdermal delivery of protein could be mediated by effective and nontoxic AID peptides in a neither fusion protein nor conjugation fashion. Therefore, noncovalent AID peptides may provide a useful strategy to have active proteins function in living cells and tissues in vivo.
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