Application of Elimination Voltammetry to the Resolution of Adenine and Cytosine Signals in Oligonucleotides II. Hetero-Oligodeoxynucleotides with Different Sequences of Adenine and Cytosine Nucleotides - Oligonucleotides

Application of Elimination Voltammetry to the Resolution of Adenine and Cytosine Signals in Oligonucleotides II. Hetero-Oligodeoxynucleotides with Different Sequences of Adenine and Cytosine Nucleotides
Received: 17 November 2005; Accepted: 28 December 2005
Published Online: 9 Mar 2006
Libue Trnkov? 1 *, Frantiek Jelen 2, Irena Postbieglov? 1
Electroanalysis
Volume 18, Issue 7
Wiley InterScience
Copyright ? 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
1Department of Theoretical and Physical Chemistry, Faculty of Science, Masaryk University, Kotl?sk? 2, CZ-611 37 Brno
2Institute of Biophysics, Academy of Sciences of the Czech Republic, CZ-612 65 Brno, Czech Republic

email: Libue Trnkov? (libuse@chemi.muni.cz)
*Correspondence to Libue Trnkov?, 1Department of Theoretical and Physical Chemistry, Faculty of Science, Masaryk University, Kotl?sk? 2, CZ-611 37 Brno
Keywords
Reduction of adenine and cytosine in oligonucleotides ? Hanging mercury drop electrode (HMDE) ? Elimination voltammetry with linear scan (EVLS) ? Hetero-oligodeoxynucleotides
Abstract
Elimination voltammetry with linear scan (EVLS) was applied to the resolution of reduction signals of adenine (A) and cytosine (C) residues in short synthetic hetero-oligodeoxynucleotides (ODNs) with different sequences of A and C. The EVLS evaluation required linear sweep voltammograms measured on a hanging mercury drop electrode (HMDE) at different scan rates. Compared to linear sweep voltammetry (LSV) and usual voltammetric methods the EVLS is capable of resolving the overlapped A and C signals, specifically by using the elimination function which eliminates the charging and kinetic currents (Ic, Ik) and conserves the diffusion current (Id). Since for an adsorbed electroactive substance this elimination function yields a well readable peak-counterpeak signal, the adsorptive stripping (AdS) procedure was favorably used. The adsorption of ODNs was carried out at -0.1 V for accumulation time of 120 s under stirring. It was found that heights and potentials of LSV signals were affected by ODNs concentrations, pH, scan rates, time of accumulation, and stirring speed during the adsorption. While on LSV curves the only one reduction peak of A and C residues was observed in all ODNs, the EVLS yielded two separated peaks in dependence on A, C sequences and pH. Our results showed that the EVLS in connection with the AdS procedure is a useful tool for qualitative and quantitative studies of short ODNs and a promising sensitive method for the development of electrochemical sensor following the ODN sequences.
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Digital Object Identifier (DOI)
10.1002/elan.200503447 About DOI
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