Antiviral applications of RNAi. - RNAi

Antiviral applications of RNAi.
April 8, 2006
Morris KV, Rossi JJ.
PubMed
RNA interference (RNAi) is a natural mechanism by which small interfering RNA (siRNA) operates to specifically and potently downregulate the expression of a target gene. This downregulation has been thought to predominantly function at the level of mRNA, as post-transcriptional gene silencing. The discovery that siRNAs can suppress gene expression at the level of transcription, that is, transcriptional gene silencing, has created a major paradigm shift in mammalian RNAi. These findings significantly broaden the role that RNA, specifically siRNA and potentially microRNA, plays in the regulation of gene expression, as well as the breadth of potential siRNA target sites. Indeed, the specificity and simplicity of design makes the use of siRNAs to target and suppress virtually any gene of interest a realized technology. Furthermore, since siRNAs are small nucleic acid reagents, they are unlikely to elicit an immune response, theoretically making them good therapeutics. The development, delivery and potential therapeutic use of antiviral siRNAs in treating viral infections and emerging viral threats are reviewed.
Intracellular delivery of poly(ethylene glycol) conjugated antisense oligonucleotide using cationic lipids by formation of self-assembled polyelectrolyte complex micelles
Engineering Village 2
2006 Elsevier Inc.
Accession number: 064310193470

Title: Intracellular delivery of poly(ethylene glycol) conjugated antisense oligonucleotide using cationic lipids by formation of self-assembled polyelectrolyte complex micelles

Authors: Jeong, Ji Hoon; Kim, Sun Hwa; Kim, Sung Wan; Park, Tae Gwan

Author affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, South Korea

Serial title: Journal of Nanoscience and Nanotechnology

Abbreviated serial title: J. Nanosci. Nanotechnol.

Volume: v 6

Issue: n 9-10

Issue date: September/October 2006

Publication year: 2006

Pages: p 2790-2795

Language: English

ISSN: 1533-4880

Document type: Journal article (JA)

Publisher: American Scientific Publishers, Stevenson Ranch, CA 9138-1439, United States

Abstract: A polyelectrolyte complex (PEC) micelle-based antisense oligodeoxynucleotide (ODN) delivery system was designed to overcome intrinsic limitations of cationic lipid-mediated gene transfer. Cationic lipid (Lipofectamine [trademark] , LF) and ODN conjugated poly(ethylene glycol) (PEG) were ionically complexed to form self-assembled spherical PEC micelles. They have a distinctive structural feature: a charge-neutralized core surrounded by highly flexible PEG corona. The PEC micelles could be visualized as a nano-sized sphere by atomic force microscopy (AFM). The DNA/LF PEC micelles exhibited far improved transfection efficiency compared to those of conventional lipoplex formulations (ODN/LF) in the presence of serum. They showed enhanced cellular uptake followed by rapid nuclear localization of ODN in human epithelial carcinoma (KB) cells. In addition, anti-proliferative activity of c-raf gene-directed antisense ODN was almost fully maintained in KB cells in the presence of serum. Copyright ? 2006 American Scientific Publishers. All rights reserved.

Number of references: 31

Ei main heading: Polyethylene glycols

Ei controlled terms: Lipids - Genes - Micelles - Atomic force microscopy - Cells

Uncontrolled terms: Oligonucleotide - Polyelectrolyte Complex Micelles - Non-Viral Gene Delivery - Human epithelial carcinoma

Ei classification codes: 815.1.1 Organic Polymers - 804.1 Organic Compounds - 461.2 Biological Materials - 801.3 Colloid Chemistry - 741.3 Optical Devices & Systems

Treatment: Theoretical (THR); Experimental (EXP)

DOI: 10.1166/jnn.2006.414

Database: Compendex

Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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