Affinity liquid chromatography and capillary electrophoresis of seminal plasma proteins
Affinity liquid chromatography and capillary electrophoresis of seminal plasma proteins
Engineering Village 2
2006 Elsevier Inc.
Accession number: 06259945494
Title: Affinity liquid chromatography and capillary electrophoresis of seminal plasma proteins
Authors: Varilova, Tereza; Semenkova, Hana; Horak, Pavel; Madera, Milan; Pacakova, Vera; Ticha, Marie; Stulik, Karel
Author affiliation: Department of Analytical Chemistry, Faculty of Science, Charles University, 128 43 Prague 2, Czech Republic
Serial title: Journal of Separation Science
Abbreviated serial title: J. Sep. Sci.
Volume: v 29
Issue: n 8
Issue date: May 2006
Publication year: 2006
Pages: p 1110-1115
Language: English
ISSN: 1615-9306
CODEN: JSSCCJ
Document type: Journal article (JA)
Publisher: Wiley-VCH Verlag, Weinheim, D-69451, Germany
Abstract: Interactions of boar, bull, and human seminal plasma proteins with heparin and phosphorylcholine were studied by affinity LC using heparin immobilized to a Toyo-pearl support. A step gradient elution from 0.15 to 1.50 M NaCl was employed to elute the seminal plasma proteins. Relative amounts of the heparin-binding fraction of seminal plasma proteins (H+) in seminal plasma of three species were determined. Further on, the fraction of seminal plasma proteins interacting with phosphorylcholine-binding proteins (P+) was evaluated. P+ proteins were not found in human seminal plasma and their highest amount was present in bull seminal plasma. ACE method was developed for separation of seminal plasma proteins. Various capillaries and separation conditions were tested; the best resolution was obtained in a bare-silica capillary, with a micellar system consisting of a 0.02 M borate buffer and 0.05 M SDS pH 10.0. The optimized conditions were applied to the identification of the components in boar plasma. ? 2006 Wiley-VCH Verlag GmbH and Co. KGaA, Weinheim.
Number of references: 22
Ei main heading: Liquid chromatography
Ei controlled terms: Affinity chromatography - Electrophoresis - Proteins - Plasmas - Silica - Micelles - pH effects - Composition effects
Uncontrolled terms: Affinity liquid chromatography - Capillary electrophoresis - Phosphorylcholine
Ei classification codes: 802.3 Chemical Operations - 801.4.1 Electrochemistry - 804.1 Organic Compounds - 932.3 Plasma Physics - 801.3 Colloid Chemistry - 801.1 Chemistry, General
Treatment: Experimental (EXP)
DOI: 10.1002/jssc.200500405
Database: Compendex
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