A novel optical immunosensing system based on measuring surface enhanced light scattering signals of solid supports
A novel optical immunosensing system based on measuring surface enhanced light scattering signals of solid supports
Engineering Village 2
2006 Elsevier Inc.
Accession number: 06189847918
Title: A novel optical immunosensing system based on measuring surface enhanced light scattering signals of solid supports
Authors: Zhao, Hua Wen; Huang, Cheng Zhi; Li, Yuan Fang
Author affiliation: Laboratory of Molecular Chemistry and Chembiology, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China
Serial title: Analytica Chimica Acta
Abbreviated serial title: Anal. Chim. Acta
Volume: v 564
Issue: n 2
Issue date: Apr 6 2006
Publication year: 2006
Pages: p 166-172
Language: English
ISSN: 0003-2670
CODEN: ACACAM
Document type: Journal article (JA)
Publisher: Elsevier, Amsterdam, 1000 AE, Netherlands
Abstract: An optical immunosensor based on measuring the surface enhanced light scattering (SELS) signals is developed by simultaneously scanning the excitation and the emission monochromators of a common spectrofluorometer. The formation of silanization on solid surface of glass slides and the binding of antibody with antigen in series have been confirmed by measuring the SELS signals. When 100 ?g ml-1 goat anti-human IgG, goat anti-rabbit IgG and rabbit anti-human IgG are immobilized on the solid surface, human IgG and rabbit IgG in the range of 0.1-100 ?g ml-1 and human IgG in the range of 0.1-10 ?g ml-1 could be detected, and the lowest detectable concentration could, respectively, reach 10, 50 and 10 ng ml-1. This label-free assay is more rapid and simpler than those conventional immunoassay methods. Under optimal experimental conditions, the sensor based on SELS has a high specificity with corresponding antigen (Ag), and other co-existing proteins in solution, for example, such as bovine serum albumin (BSA) and human serum albumin (HSA) do not show interference effect. Regenerated simply by rinsing in carbamide solution, the sensor could achieve three assay cycles without great loss of sensitivity. ? 2006 Elsevier B.V. All rights reserved.
Number of references: 45
Ei main heading: Biosensors
Ei controlled terms: Immunology - Bioelectric potentials - Light scattering - Monochromators - Antigens - Antibodies
Uncontrolled terms: Surface enhanced light scattering (SELS) signals - Immunosensor - Antigen (Ag) - Antibody (Ab)
Ei classification codes: 461.8 Biotechnology - 461.9.1 Immunology - 461.1 Biomedical Engineering - 741.1 Light/Optics - 741.3 Optical Devices & Systems
Treatment: Theoretical (THR)
DOI: 10.1016/j.aca.2006.01.096
Database: Compendex
Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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