Why are proteins charged? Networks of charge-charge interactions in proteins measured by charge ladders and capillary electrophoresis
Why are proteins charged? Networks of charge-charge interactions in proteins measured by charge ladders and capillary electrophoresis
Engineering Village 2
2006 Elsevier Inc.
Accession number: 063010030400
Title: Why are proteins charged? Networks of charge-charge interactions in proteins measured by charge ladders and capillary electrophoresis
Authors: Gitlin, Irina; Carbeck, Jeffrey D.; Whitesides, George M.
Author affiliation: Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, United States
Serial title: Angewandte Chemie - International Edition
Abbreviated serial title: Angew. Chem. Int. Ed.
Volume: v 45
Issue: n 19
Issue date: May 5 2006
Publication year: 2006
Pages: p 3022-3060
Language: English
ISSN: 1433-7851
CODEN: ACIEAY
Document type: Journal article (JA)
Publisher: Wiley-VCH Verlag, Weinheim, D-69451, Germany
Abstract: Almost all proteins contain charged amino acids. While the function in catalysis or binding of individual charges in the active site can often be identified, it is less clear how to assign function to charges beyond this region. Are they necessary for solubility? For reasons other than solubility? Can manipulating these charges change the properties of proteins? A combination of capillary electrophoresis (CE) and protein charge ladders makes it possible to study the roles of charged residues on the surface of proteins outside the active site. This method involves chemical modification of those residues to generate a large number of derivatives of the protein that differ in charge. CE separates those derivatives into groups with the same number of modified charged groups. By studying the influence of charge on the properties of proteins using charge ladders, it is possible to estimate the net charge and hydrodynamic radius and to infer the role of charged residues in ligand binding and protein folding. ? 2006 Wiley-VCH Verlag GmbH and Co. KGaA.
Number of references: 268
Ei main heading: Proteins
Ei controlled terms: Electrophoresis - Catalysis - Chemical modification - Electrostatics
Uncontrolled terms: Capillary electrophoresis - Charge networks - Charge regulation - Electrostatic interactions
Ei classification codes: 804.1 Organic Compounds - 701.1 Electricity: Basic Concepts & Phenomena - 802.2 Chemical Reactions
Treatment: Experimental (EXP)
DOI: 10.1002/anie.200502530
Database: Compendex
Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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