Time-resolved chemiluminescent analysis of hydralazine in pharmaceuticals
Time-resolved chemiluminescent analysis of hydralazine in pharmaceuticals
Engineering Village 2
2006 Elsevier Inc.
Accession number: 06019628517
Title: Time-resolved chemiluminescent analysis of hydralazine in pharmaceuticals
Authors: Murillo Pulgarin, Jose A.; Fernandez Lopez, Pablo; Hoyas Nuno, Pilar
Author affiliation: Department of Analytical Chemistry and Foods Technology, University of Castilla-La Mancha, 13071 Ciudad Real, Spain
Serial title: Analytical and Bioanalytical Chemistry
Abbreviated serial title: Anal. Bioanal. Chem.
Volume: v 384
Issue: n 2
Issue date: January 2006
Publication year: 2006
Pages: p 423-430
Language: English
ISSN: 1618-2642
CODEN: ABCNBP
Document type: Journal article (JA)
Publisher: Springer Verlag, Heidelberg, D-69121, Germany
Abstract: A new analytical method is proposed for determination of hydralazine (HZ) in pharmaceuticals-measurement of the chemiluminescence (CL) emitted after reaction with phosphoric-acidified KMnO4. The novelty of this method is the recording of the whole CL-time profile. Such a recording is possible by use of a CL-detector operating in tandem which enables the reactants to be mixed in the measurement cell only and, therefore, the CL is reaction monitored from beginning. At the precise time the pump is stopped signal recording is triggered and so CL evolution is recorded completely. The optimum chemical conditions for the determination were 0.8 mol L-1 formaldehyde, 0.3 mmol L -1 KMnO4, 4.0 mol L-1 H3PO 4, and a total flow of 0.37 mL s-1. Two calibration graphs were plotted, CL intensity and area under the profile curve against HZ concentration. Exhaustive statistical analysis provided very interesting results, for example, accordance with Clayton's theory, detection limit below 0.2 ?g mL-1, and linear calibration ranges from 0.2 to 5.0 ?g mL-1. This method was successfully applied to the determination of HZ in pharmaceuticals. Because they are usually formulated in association with diuretics and ?-blockers, the method was used for analysis of HZ in pharmaceuticals that contained either HZ only or HZ with other hypotensive substances. Obtained and nominal content were approximately the same and experimental Student t values indicated there were no significant differences between the values.
Number of references: 34
Ei main heading: Nitrogen compounds
Ei controlled terms: Chemiluminescence - Drug products - Formaldehyde - Chemical modification - Potassium compounds - Inorganic acids
Uncontrolled terms: Hydralzine - Apressinum - Time-resolved chemiluminescence - Hydralzine hydrochloride
Ei classification codes: 804.2 Inorganic Compounds - 741.1 Light/Optics - 802.2 Chemical Reactions - 461.6 Medicine - 804.1 Organic Compounds
Treatment: Experimental (EXP)
DOI: 10.1007/s00216-005-0191-6
Database: Compendex
Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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